Table 2.

Effect of metal ions, surfactants, and other chemicals on the esterolytic activity of EstDZ3.

Metal ion or chemical agent	Concentration	Relative activity (%)
None	–	100.0 ± 4.1
K+	1 mM	93.1 ± 6.7
Mn2+	1 mM	101.4 ± 6.2
Ca2+	1 mM	78.4 ± 3.0
Zn2+	1 mM	45.7 ± 5.7
Li2+	1 mM	90.8 ± 3.7
Mg2+	1 mM	90.1 ± 4.2
Na+	1 mM	90.4 ± 5.5
Fe3+	1 mM	78.3 ± 3.5
Cu2+	1 mM	40.6 ± 3.4
EDTA	1 mM	96.7 ± 5.1
PMSF	1 mM	33.8 ± 5.8
Triton X-100	1% (v/v)	67.4 ± 1.5
Tween 20	1% (v/v)	70.5 ± 5.4
Tween 80	1% (v/v)	73.2 ± 4.7
SDS	1% (w/v)	21.4 ± 2.5