Skip to main content
. 2004 Jul 27;101(32):11797–11802. doi: 10.1073/pnas.0403165101

Fig. 1.

Fig. 1.

Leptin stimulates the hypothalamic content of αMSH in mice. (A) Total αMSH levels in the hypothalamus (HYP) and cortex (CTX) of C57BL/6J mice (black bars) and Wistar rats (white bars). (Inset) Immunohistochemistry demonstrates αMSH-producing POMC neurons in the arcuate nucleus (ARC) of rat hypothalamus (arrows) and the lack of αMSH in the cortex. ME, median eminence; 3V, third ventricle. (Scale bars = 200 μm.) (B) Hypothalamic (HYP) αMSH levels in ob/ob mice with or without 3 h of leptin treatment. (C) Hypothalamic (HYP) αMSH in C57BL/6J mice after 3 days of leptin and PBS treatment and in pair-fed control mice. Leptin reduced food intake with 0.9 ± 0.2 g/day per mouse. Data in B and C are means ± SE of five animals. Significant differences were determined by Student's t test. **, P < 0.005; ***, P < 0.0005.