Fig. 3.

Leptin stimulates the activity of an αMSH N-acetyltransferase in the hypothalamus of C57BL/6J and ob/ob mice. Hypothalamic protein extracts from mice injected with either leptin or PBS (3 h) were incubated with purified Des-αMSH and acetyl CoA for 10 min. Samples were applied to an HPLC system, and fractions were measured by RIA. The condition of the HPLC analysis was changed so that elution times were different, compared with those shown in Fig. 2. (A) Results from C57BL/6J mice. (B) Results from ob/ob mice. Data are means ± SE of three to four animals in each group.