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. 2004 Jul 27;101(32):11797–11802. doi: 10.1073/pnas.0403165101

Fig. 6.

Fig. 6.

Act-αMSH is more stable than Des-αMSH. (A) Fifty nanomolar Des-αMSH or Act-αMSH was incubated with cells for up to 5 h, and residual peptide amounts were measured by RIA. (B) Fifty nanomolar Des-αMSH or Act-αMSH was incubated in pure medium (black bars), in medium with 293T cells (gray bar), or in medium with 293T cells expressing MC4R (white bar) for 5 h, and residual peptides were measured by RIA. Data are means ± SE of results obtained in triplicate. (C) Fifty nanomolar Des-αMSH or Act-αMSH was incubated with brain lysates from C57BL/6J mice at 37°C, and residual amounts of peptides were measured by RIA. Data are means ± SE of results obtained in triplicate. Significant differences were determined by ANOVA and Student's t test for post hoc analysis. *, P < 0.05; ***, P < 0.0005. (D) Saline or 0.3 or 3 nmol of Des-αMSH or Act-αMSH was injected into the third ventricle of Wistar rats, and residual amounts in the hypothalamus were measured by RIA 30 min postinjection of 3 nmol and 15 min postinjection of 0.3 nmol.