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. 2016 Nov 16;4:131. doi: 10.3389/fcell.2016.00131

Figure 1.

Figure 1

Protection of BALB/c mice upon immunization with rexPy + CpG-ODN and lethal challenge is spleen-dependent. (A) Survival curve and (B) daily time parasitemia after 5 × 105 P. yoelii 17XL infections of groups of BALB/c previously immunized with subcutaneously (s.c.) with CpG-ODN-1826 plus rexC (n = 3) or rexPy (n = 3). Non-immunized (NI) mice (n = 3) were untreated. Differences in the survival curves between NI, I rexC and I rexPy (P < 0.05) are statistically significant [Log-rank (Mantel-Cox Test)]. Parasitemia is represented as mean + SD of animals with presence of parasite in blood. I rexC mice were recovered (no parasite in blood) at days 13,23 and 37. (C) Survival curve and (D) daily time-course parasitemia after 5 × 105 P. yoelii 17XL infections of groups of splenectomized BALB/c mice previously immunized subcutaneously (s.c.) with CpG-ODN plus rexC (n = 3) or rexPy (n = 3). Non-immunized (NI) mice (n = 3) were untreated. Parasitemia is represented as mean + SD of animals with presence of parasite in blood. (E) Survival curve and (F) daily time-course parasitemia after 5 × 105 P. yoelii 17XL infections of groups of animals previously transferred with splenocytes from rexC (t sprexC, n = 4) and rexPy (t sp rexPy, n = 4) immunized animals. Non-transferred (nt) mice (n = 4) were untreated. Differences in the survival curves between nt and t sp rexPy (P < 0.05) and between t sp rexC and t sp rexPy (P < 0.05) are statistically significant [Log-rank (Mantel-Cox Test)]. Parasitemia is represented as mean + SD of animals with presence of parasite in blood. I rexC mice were recovered (no parasite in blood) at days 23,33 (n = 2) and 39.