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. 2016 Nov 16;4:131. doi: 10.3389/fcell.2016.00131

Figure 3.

Figure 3

Phenotypic analysis of T-cells after immunization with exosomes. BALB/c mice were immunized with rexC or rexPy plus CpG-ODN-1826. Two weeks after the second immunization spleen cells were obtained. Non-immunized mice (NI) were untreated. A group of mice was infected with P. yoelii 17X and spleen cells were obtained 25 days post infection (Py p.i). Spleen cells were labeled with the panel of antibodies described in material and methods and analyzed in a LSR Fortessain order to differentiate between the different T cell memory populations: effector memory T cells (CD62L−CD44+), central memory (CD62L+CD127+), effector memory (CD62L−CD127+) and activated effector T cells (CD62L−CD127−). (A) Quantification of the percentage of different population subsets from CD4+cells. (B) Quantification of the percentage of different subsets from CD8+cells. Data correspond to the mean ± standard error of 9 mice per group from three different experiments. Percentages are evaluated with Kruskal-Wallis test and vs. non-immunized group (Dunn's post hoc test). (*P < 0.05, ***P < 0.001). Differences between I rexC and I rexPy groups are analyzed with Mann-Whitney test (◦P < 0.05, ◦◦◦P < 0.001).