Table 1.
List of strains and plasmids used in this study.
| Strain/plasmid | Description* | Source |
|---|---|---|
| S. PNEUMONIAE | ||
| D39 | Serotype 2 strain | Laboratory of P. Hermans |
| MA700 | D39 ΔnagA; TrmR | This study |
| MA701 | D39 ΔnagB; SpecR | This study |
| MA702 | D39 ΔglmS | This study |
| MA703 | D39 ΔbgaA::PnagA-lacZ; TetR | This study |
| MA704 | D39 ΔbgaA::PnagB-lacZ; TetR | This study |
| MA705 | D39 ΔbgaA::PglmS-lacZ; TetR | This study |
| MA706 | D39 ΔbgaA::PmanL-lacZ; TetR | This study |
| MA707 | D39 ΔbgaA::PnagA-M-lacZ; TetR | This study |
| MA708 | D39 ΔbgaA::PnagB-M-lacZ; TetR | This study |
| MA709 | D39 ΔbgaA::PglmS1-M-lacZ; TetR | This study |
| MA710 | D39 ΔbgaA::PglmS3-M-lacZ; TetR | This study |
| MA203 | D39 ΔbgaA::PnanE-lacZ; TetR | Afzal et al., 2015b |
| E. COLI | ||
| EC1000 | Laboratory collection | |
| PLASMIDS | ||
| pPP2 | AmpR TetR; promoter-less lacZ. For replacement of bgaA with promoter lacZ fusion. Derivative of pPP1 | Halfmann et al., 2007 |
| pORI280 | ErmR; ori+ repA−; deletion derivative of pWV01; constitutive lacZ expression from P32 promoter | Leenhouts et al., 1998 |
| pMA700 | pORI280 carrying glmS deletion | This study |
| pMA701 | pPP2 PnagA-lacZ | This study |
| pMA702 | pPP2 PnagB-lacZ | This study |
| pMA703 | pPP2 PglmS-lacZ | This study |
| pMA704 | pPP2 PmanL-lacZ | This study |
| pMA705 | pPP2 PnagA-M-lacZ | This study |
| pMA706 | pPP2 PnagB-M-lacZ | This study |
| pMA707 | pPP2 PglmS1-M-lacZ | This study |
| pMA708 | pPP2 PglmS3-M-lacZ | This study |
*
AmpR,
SpecR, TetR, and TrmR confer ampicillin, spectinomycin, tetracycline and trimethoprim resistance gene, respectively.