Table 2.
List of primers used in this study.
| Name | Nucleotide sequence (5′ 3′)* |
Restriction site |
|---|---|---|
| nagA-R | CATGGGATCCGTCCACAAGTTCCAAGTAACC | BamHI |
| nagA-F | CATGGAATTCGCAGACAGCTCAAGACAAGC | EcoRI |
| nagA-F-M | CATGGAATTCTATCTCCAAAAAATAGGTCGCTGTCATTTACAAAT | EcoRI |
| nagB-R | CATGGGATCCGCAACTTTTCCACCTTGAACTTGG | BamHI |
| nagB-F | CATGGAATTCGGGCAATCAATTCCTCTGGC | EcoRI |
| nagB-F-M | CATGGAATTCCGTTTTCACTTGACAAAAATTGGTCGCTGTCATATAATAA | EcoRI |
| glmS-R | CATGGGATCCCAACACCAACAATTCCACAC | BamHI |
| glmS-F | CATGGAATTCCGTCGTCTGAAGAAATCAGG | EcoRI |
| manL-F | CATGGAATTCCAGTAGAAGATGCTGTTG | EcoRI |
| manL-R | CATGGGATCCTGACTGATGAATACCC | BamHI |
| glmS1-F-M | CATGGAATTCACAGGAGCTTAATTTGAACGCTGTCAATTTTTACTC | EcoRI |
| glmS3-R-M | CATGGGATCCCACATAGTATATACGACACAGGCAAGCTGTGCTTTCTCCTTAAAATTGGGCGCGTCTAATTCA | BamHI |
| nagA-1 | GACGGTGGTCATTGCGACTG | – |
| nagA-2 | GCATAGGCGCGCCCCTCGACGAACTCCGTGTG | AscI |
| nagA-3 | CGATTGCGGCCGCGGTAGCAACCTACCTAGATGG | NotI |
| nagA-4 | CGTAGATATTCAGCCTGCATACC | – |
| nagB-1 | GGGTGTCGTTCATGACAAGGG | – |
| nagB-2 | GCATAGGCGCGCCGCTACTTCCTGTCGCAAGTCC | AscI |
| nagB-3 | CGATTGCGGCCGCGCAGATGCTGAAGCGCTTAGC | NotI |
| nagB-4 | CCATAGACAATGTCTAGTCTAAGC | – |
| glmS-1 | TGCTCTAGAGGTCATCTTCGTGAACTTCACCG | XbaI |
| glmS-2 | CCGCAGAATCATAGCCACGG | – |
| glmS-3 | GCTATGATTCTGCGGCGACTGTACACCCTTACCTCTC | – |
| glmS-4 | GAAGATCTCCAGGACAATCTCTGGGGC | BglII |
| Spec-R | GCTAAGCGGCCGCACTAAACGAAATAAACGC | NotI |
| Spec-F | GCTATGGCGCGCCCTAATCAAAATAGTGAGGAGG | AscI |
| Trmp-R | GCATGCGGCCGCGTTACGACGCGCATAGACGG | AscI |
| Trmp-F | GCATGGCGCGCCGGATTTTTGTGAGCTTGGA | NotI |
*
The underlined sequences represent the respective restriction sites.