Table 2.

List of primers and reaction conditions for candidate barcodes.

DNA region	Primer pairs	Primer sequences (5′-3′)	Thermocycling conditions
rbcL	rbcl1F	ATGTCACCACAAACAGAAAC	94°C 5 min, 36 cycles (94°C 40 s, 52°C 40 s, 72°C 1 min), 72°C 10 min
	rbcl724R	TCGCATGTACCTGCAGTAGC
matK	matK3Fkim	CGTACAGTACTTTTGTGTTTACGAG	94°C 5 min, 36 cycles (94°C 40 s, 52°C 40 s, 72°C 1 min), 72°C 10 min
	matK1Rkim	ACCCAGTCCATCTGGAAATCTTGGTTC
trnS-trnG	trnS-trnGF	GCCGCTTTAGTCCACTCAGC	94°C 5 min, 36 cycles (94°C 40 s, 56°C 40 s, 72°C 1 min), 72°C 10 min
	trnS-trnGR	GAACGAATCACACTTTTACCAC
ITS	ITS4	TCCTCCGCTTATTGATATG	94°C 5 min, 36 cycles (94°C 40 s, 52°C 40 s, 72°C 1 min), 72°C 10 min
	ITS5	GGAAGGAGAAGTCGTAACAAGG