Table 3.

Design for Youden trial, in all cases n = 3, uppercase letter indicates high level, lowercase letters indicate low level.

	Temperature (A/a)	Sonication (B/b)	Light (C/c)	Oxidation (D/d)	Solvent (E/e)
Run 1	4°C (a)	0 min (b)	Green (c)	Nitrogen (d)	100% MeOH (e)
Run 2	40°C (A)	0 min	Green	– (D)	10% MeOH (E)
Run 3	4°C	30 min (B)	Green	–	10% MeOH
Run 4	40°C	30 min	Green	Nitrogen	100% MeOH
Run 5	4°C	0 min	White (C)	Nitrogen	100% MeOH
Run 6	40°C	0 min	White	–	10% MeOH
Run 7	4°C	30 min	White	–	10% MeOH
Run 8	40°C	30 min	White	Nitrogen	100% MeOH