Table 5.
Summary of retention time, and instrument and method limits of detection and quantification for all analytes investigated.
| Analyte | Retention time (min) | Instrument LOD (ng/mL) | Method LOD (μg/g) | Instrument LLOQ (ng/mL) | Method LLOQ (μg/g) | Instrument ULOQ (μg/mL) | Method ULOQ (mg/g) |
|---|---|---|---|---|---|---|---|
| Serotonin | 1.057 | 24.4 | 1.62 | 97.7 | 6.51 | 25 | 1.67 |
| Tryptophan | 1.045 | 6.10 | 0.407 | 24.4 | 1.62 | 25 | 1.67 |
| Tryptamine | 2.752 | 1.52 | 0.101 | 6.1 | 0.407 | 6.25 | 0.42 |
| N-acetylserotonin | 2.480 | 6.10 | 0.407 | 24.4 | 1.62 | 25 | 1.67 |
| Melatonin | 3.369 | 0.093 | 0.00617 | 0.38 | 0.0254 | 6.25 | 0.42 |
LOD—limit of detection, LLOQ—lower limit of quantification, RT—retention time, ULOQ—upper limit of quantification.