Fig. 3.
Effects of Zantrins on the ultrastructure of FtsZ polymers. Assembly reactions containing 2 μM FtsZEc in buffer E were preincubated with varying concentrations of Zantrins or with 5% DMSO for 3 min, before adding 1 mM GTP (pH ≈7.0). After 2 min at 25°C, 10-μl aliquots were applied to glow-discharged carbon-coated copper grids (400 mesh size), which were stained with 0.75% uranyl formate. The filaments were photographed by using a Philips 401 (Eindhoven, The Netherlands) electron microscope at ×50,000. The arrow in F indicates clustered doublets of FtsZ PFs. An adjacent FtsZ bundle is also visible. (Bar = 100 nm.)