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. 2004 Jul 28;101(32):11833–11838. doi: 10.1073/pnas.0404163101

Fig. 5.

Fig. 5.

Immunologic analysis of Emm3.1 and Emm3.2. (A) Emm3 synthetic peptides used to immunize rabbits. M3.1 and M3.2 peptides correspond to the first 24 and 28 aa of mature Emm3.1 and Emm3.2, respectively. The first four amino acids of mature Emm3.1, which are duplicated in Emm3.2, are shown in red. (B) ELISA reactivity of rabbit antibodies with Emm3.1 and Emm3.2 peptides. Affinity-purified rabbit anti-Emm3 peptide antibodies were diluted 1:80,000. Underlined amino acids correspond to the peptides used to immunize rabbits. (C) Human PMN phagocytosis studies. Strains MGAS3392 (Emm3.1) and MGAS9887 (Emm3.2) were opsonized with either rabbit anti-M3.1 or anti-M3.2 antibodies at the indicated concentrations and incubated with human PMNs. Values are the mean of five to six independent assays using PMNs obtained from different donors. Error bars show the standard error.