Fig. 1.
Expression pattern of gprk1. (A) Northern blot analysis. mRNAs from WT heads (WH), sine oculis heads (SH), and WT bodies (WB) were probed with 32P-labeled gprk1 and rp49 DNAs. RNA markers (kb) are indicated. (B) Western blot analysis. α-GRK2 antibodies were used to probe a blot containing extracts from WT bodies (WB), WT heads (WH), sine oculis heads (SH), and heads from rdgA flies. Parallel blots were probed with α-TRP and α-tubulin antibodies. Size markers (kDa) are indicated. (C) Spatial distribution of gprk1 RNA in adult fly heads. In situ hybridizations to frontal sections were performed with antisense and sense gprk1 probes. A phase-contrast image of the same section hybridized with the gprk1 sense probe is shown. B, brain; M, medulla; L, lamina; R, retina.