CB1 receptor protein is reduced in male rats. (Upper) Western blot showing the expression of CB1 receptor protein in MBH from OVX, OVX-E, and normal male rats. (Lower) Optical density of CB1 receptor expressed in MBH of OVX, OVX-E, and male rats (each column represents the mean of four separate determinations). **, P < 0.01 and *, P < 0.05 vs. male rats.