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. 2016 Nov 16;6:37222. doi: 10.1038/srep37222

Figure 1. Phenotypic and elemental analyses of the nramp1-1 irt1-1 double mutant.

Figure 1

(a) NRAMP1 and IRT1 expression in nramp1-1, irt1-1 and nramp1-1 irt1-1 double mutant as compared to wild type Col-8 plants. RT-qPCR on RNA extracted from roots of 4 week-old plants grown in hydroponic cultures under standard (25 μM Fe, 20 μM Mn) conditions. Transcripts levels are expressed relative to those of the reference gene ACTIN ± sd. (n = 3 technical replicates). nd, non-detectable. Two biological replicates of this experiment are shown in Supplementary Fig. S1. (b) 21 day-old plants grown as in (a). (c) Fresh biomass, (d) photosynthetic parameters (maximal quantum yield Fv/Fm and effective quantum yield фII), (e) and (f) respectively shoot Mn and Fe contents, (g) and (h) respectively root Mn and Fe contents of 4 week-old plants grown as in (a). (c to h) share colour code legends. Mean ± sd. (n = 8 to 11 plants in (c); n = 4 to 5 plants in (d), n = 3 to 4 replicates of 8 to 10 plants each in (e to h)). Asterisks indicate values statistically different from those of Col plants (Student T-test, *P-value < 0.05, **P-value < 0.01, ***P-value < 0.001).