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. 2016 Nov 16;6:37222. doi: 10.1038/srep37222

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(a) and (b) Perls staining of entire roots from 15 day-old wild type (WT) and nramp1-1 irt1-1 seedlings grown in vitro in standard conditions. (c) and (d) Perls-DAB staining of thin root sections of WT and nramp1-1 irt1-1 seedlings grown as in (a) A blue arrow in panel (d) highlights the interruption of the Perls-DAB labelling by the casparian strip.