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. 2016 Nov 16;6:37222. doi: 10.1038/srep37222

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Copyright © 2016, The Author(s)

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(a) Picture of 24 day-old nramp1-1 irt1-1 mutants grown in hydroponic medium containing 20 μM Mn and a range of Fe supply (25 μM, 500 μM Fe, 1 mM Fe, top lane) or 25 μM Fe and a range of Mn supply (20 μM, 1 mM Mn, 2 mM Mn, bottom lane). The inset in the bottom right picture shows a close up of the leaf necrotic lesions. (b) and (c) respectively Fe and Mn concentration in shoots of plants grown as in (a). Wild type plants (black), nramp1-1 (dark grey), irt1-1 (light grey) and nramp1-1 irt1-1 mutants (stripes). Means ± sd. (n = 3 replicates of 8 to 12 plants each). (d) and (e) Respectively FRO2 and FER1 expression in roots of the plants shown in (b) and (c). Transcripts levels were measured by qRT-PCR relative to those of the reference gene ACTIN ± s.d. (n = 3 technical replicates).