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. 2016 Nov 16;6:37297. doi: 10.1038/srep37297

Figure 2. FTY720-P significantly represses TNF-induced IL-6 and IL-8 mRNA expression and protein secretion.

Figure 2

A549 cells were pretreated with 2.5 μM FTY720-P for 6 h, compared to vehicle-treated controls. Cells were treated with vehicle or TNF (4 ng/ml) and then (A,C) IL-6 and IL-8 mRNA expression (results expressed as fold increase compared to vehicle-treated cells at 0 h) and (B,D) IL-6 and IL-8 protein secretion measured (results expressed as a percentage of TNF-induced cytokine secretion at 24 h: i.e. (B) 1,769.0 ± 517.6 pg/ml and (D) 56,254.8 ± 805.2 pg/ml) at the indicated times. Statistical analysis was performed using two-way ANOVA then Bonferroni’s post-test (where * denotes a significant effect of TNF compared to vehicle-treated cells, and § denotes significant repression of TNF-induced cytokine production by FTY720-P (P < 0.05)). Data are mean + SEM values from n = 4 independent experiments.