Skip to main content
. 2016 Nov 16;6:37297. doi: 10.1038/srep37297

Figure 3. FTY720 and FTY720-P significantly repress S1P-induced IL-6 and IL-8 mRNA expression and protein secretion.

Figure 3

A549 cells were pretreated with 2.5 μM FTY720 or FTY720-P for 6 h, compared to vehicle-treated controls. Cells were treated with vehicle or S1P (1 μM) and then (A,C) IL-6 and IL-8 mRNA expression (results expressed as fold increase compared to vehicle-treated cells at 0 h) and (B,D) IL-6 and IL-8 protein secretion measured (results expressed as a percentage of TNF-induced cytokine secretion at 24 h: i.e. (B) 100.5 ± 34.1 pg/ml and (D) 4,363.0 ± 241.1 pg/ml) at the indicated times. Statistical analysis was performed using two-way ANOVA then Bonferroni’s post-test (where * denotes a significant effect of S1P compared to vehicle-treated cells, and § denotes significant repression of S1P-induced cytokine production by FTY720-P (P < 0.05)). Data are mean + SEM values from n = 3 independent experiments.