Table 6. Comparative genomic analysis of the seven HAdV-7d isolates from this study.
| Region | Product | Position | Mutation in DNA |
Amino acid substitution |
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| OP01 | IP01 | IP02 | IP03 | IP04 | IP05 | IP06 | OP01 | IP01 | IP02 | IP03 | IP04 | IP05 | IP06 | |||
| L1 | Virus-associated RNA II | 10815 | — | — | — | — | — | Insertion of T | — | — | — | — | — | — | — | — |
| L4 | Hexon assembly- associated protein | 24298 | — | — | C → T | C → T | — | — | — | — | — | A → A | A → A | — | — | — |
| E3 | Non-coding region | 29947 | Insertion of T | — | — | — | — | — | — | — | — | — | — | — | — | |
| E3 | membrane protein E3 RID-alpha | 29983 | — | G → A | — | — | — | — | — | — | R → K | — | — | — | — | — |
| 30060 | — | G → A | — | — | — | — | — | — | D → N | — | — | — | — | — | ||
| 30162 | — | G → A | — | — | — | — | — | — | D → N | — | — | — | — | — | ||
| 30178 | — | G → A | — | — | — | — | — | — | R → Q | — | — | — | — | — | ||
| E4 | 34 kDa protein | 33155 | — | — | — | G → T | — | — | — | — | — | — | P → Q | — | — | — |
The reference genome is HAdV-7 strain 0901HZ/ShX/2009. Nucleic acid and amino acid sequence mutations are noted, along with their genome locations of the coding regions. “—”: no change or not applicable. Seven isolates are included: one from outpatient (OP) causing mild respiratory disease named OP01_2011, three from inpatients (IP; fatal outcome) named IP01_2010, IP02_2011 and IP03_2011, two from severe cases (required intensive care and mechanical ventilation) named IP04_2011 and IP05_2012, and one from comparatively mild respiratory disease (diagnosed with bronchitis and discharged after 4-day hospitalization) named IP06_2012.