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. 2016 Nov 16;6:37308. doi: 10.1038/srep37308

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) Transmission electron micrographs (TEMs) of cross-sections of flagella of live and demembranated L. donovani. In live (non-demembranated) Leishmania intact outer flagellar membrane (FM) is visible. In demembranated Leishmania outer flagellar membrane (FM) is absent due to extraction with 0.1% Triton. (A) axoneme and (PFR) paraflagellar rod. (b) Confocal microscopy images of live (non-demembranated) and demembranated L. donovani treated with 15 μM propidium iodide (PI). Images were captured at 100X using an oil-immersion objective.