Table 1. Comparison of beat parameters for flagellar (tip-to-base) beating of live, LRP and basic protocol reactivated flagella of L. donovani.
Percent Motility | Amplitude (μm) | Beat Frequency (Hz) | Wavelength (μm) | Flagellum Length (μm) | Wave Propagation Speed (μm/s) | |
---|---|---|---|---|---|---|
Live | 90.40 ± 1.78 | 2.35 ± 0.04 | 20.80 ± 0.57 | 10.92 ± 0.17 | 15.20 ± 0.34 | 225.98 ± 6.36 |
LRP reactivated | 77.37 ± 2.62 | 2.02 ± 0.04 | 19.29 ± 0.65 | 9.95 ± 0.15 | 14.59 ± 0.30 | 192.73 ± 7.56 |
Basic protocol reactivated | 60.24 ± 8.30 | 1.88 ± 0.05 | 13.88 ± 0.46 | 9.43 ± 0.18 | 14.24 ± 0.32 | 131.32 ± 5.25 |
Mean parameters ± SEM for flagellar beating of L. donovani. Five independent experiments were performed. 53, 57 and 50 cells were quantified for beat parameters of live, LRP reactivated and basic protocol reactivated cells respectively.