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. 2016 Oct 15;30(20):2286–2296. doi: 10.1101/gad.285361.116

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© 2016 Simonini et al.; Published by Cold Spring Harbor Laboratory Press

This article, published in Genes & Development , is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/.

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Figure 2. — ETT and IND function together to regulate target genes during gynoecium development. (A) Phylogenetic shadowing using mVISTA of a 2-kb genomic region upstream of the translational start site of the PID gene with pairwise alignments of Arabidopsis thaliana with Arabidopsis lyrata, Capsella rubella, Camelina sativa, and Brassica rapa. Regions 1 and 2 (R1 and R2) are indicated by shaded areas. (B) ChIP with the pETT::ETT-GFP line showing enrichment of a fragment containing the conserved AuxRE sites at −429 and −447. The WUS promoter was used as a positive control. Error bars show standard deviation. (*) P < 0.01; (**) P < 0.001. (C–F) In situ hybridization of PID mRNA at the apex of stage 8 gynoecia from Col-0 (C), ett-3 (D), ind-2 (E), and ett-3 ind-2 (F). (se) Sepal; (st) stamen; (stg) stigma; (sty) style; (va) valve. Bars, 50 μm.