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. 2016 Oct 15;30(20):2297–2309. doi: 10.1101/gad.290619.116

Figure 2.

Figure 2.

The Dll4in3 enhancer contains a MEF2-binding motif that is required for expression during sprouting angiogenesis. (A) Schematic representation of the binding motifs found within the mouse Dll4in3 and Dll4-12 enhancer sequences. Colored shapes represent sequences directly verified by EMSA analysis (Supplemental Fig. 2; Sacilotto et al. 2013), and gray shapes indicate consensus or near-consensus sequences that did not bind the cognate transcription factors in EMSA. (B) Schematic representation of the Dll4in3mutMEF enhancer sequence in which the MEF2-binding motif within the Dll4in3 enhancer has been mutated. (C) Representative images from Dll4in3mutMEF transgenic mice demonstrate that this enhancer does not direct endothelial expression during sprouting angiogenesis in E10 embryos, E11 hindbrains, or P6 retinas, although robust expression is detected in the arterial (a) and neuronal (n) compartments. See also Supplemental Figure 2.