Figure 1.

Treatment and tumor acquisition time line and summary of whole-genome sequencing (WGS) data analyses. (A) The time line of treatment history is indicated by the vertical lines along the green bar with names of the drug used at each approximate time point. The duration of the treatment and/or the response times are indicated by the lower horizontal arrows. Tumor biopsies were taken at each time point (red ×) with the date (orange box); sample description below, sample names in parentheses. Venn diagrams show the common somatic single-nucleotide variants (SNVs) (B) and indels (C) identified by Strelka in lung tumor (L) and lymph node (LN1) metastasis genomes. (D) The Circos plot shows some of the validated key somatic alterations identified by WGS. The outer circle depicts each chromosome and its cytobands. Genes that carry key nonsynonymous mutations (dot in second circle; black, lung tumor only; green, lymph node only; and magenta, both) or the translocation breakpoints (no dot in the second circle; dark blue, lung tumor only; orange, lymph node only; and magenta, both) are marked with their respective chromosomal positions. Copy-number variations (CNVs) are shown as histogram plots from analysis of WGS data (two inner circles of lung tumor in the outer circle, and lymph node in the inner circle; green shows copy-number gains and red shows copy-number losses). Identified interchromosomal translocation events are depicted as links inside the plots (dark blue, lung tumor; orange, lymph node; and magenta, both). WES, whole-exome sequencing; TILs, tumor-infiltrating lymphocytes.