Figure 3.

Validation of all nonsilent somatic variants identified by whole-genome sequencing (WGS) with exome and targeted sequencing and by polymerase chain reaction (PCR)/Sanger sequencing for PLAG1-ACTA2 translocation. Heatmaps show the presence of variants (green, lung tumor only; blue, lymph node only; orange, both), the absence of variants (light gray), and the presence/absence cannot be determined (white) because of low or lack of coverage at each somatic variant site in lung tumor (A) and lymph node metastases (B) as assessed by targeted Ion Torrent deep resequencing or by exome sequencing (lung tumor biopsy [LBX] only), and by PCR/Sanger sequencing (for PLAG1-ACTA2 translocation in orange). Genes carrying mutations are labeled on the left edges, and the cancer genes are marked with an asterisk (*) on the side.