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. 2016 Nov 16;6:37137. doi: 10.1038/srep37137

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Bright field image of one of the gingival tissue sections stained with haematoxylin and eosin. The selected inflamed area has been marked with a black rectangle (a). Ten spots within this area were selected in all the six tissue sections (b). For each section, the transcripts mapping back to these spots were added together based on gene annotation and averaged over the ten spots. The log2 normalized gene counts of this average spot from each region were compared between the tissue sections. Upper panel show the correlations between inflamed regions of the automated samples. The lower panel plots shows the correlations within the manually prepared samples (c).