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. 2016 Nov 16;6:37095. doi: 10.1038/srep37095

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Cell migration measured by wound healing assay in HUVECs (A) and HUV-ST (B) cells. Cells were stimulated without or with 0.733, 7.33, 73.3 and 600 ng/ml of rhFSH, or 50 ng/ml of rhVEGF used as a positive control. Upper panel shows the relative wound density/time and lower panel shows the cell migration. Pictures were taken every hour by IncuCyte ZOOM^®. Relative wound density was calculated by IncuCyte™ Chemotaxis Cell Migration Software. Each bar represents the mean ± SEM of three independent experiments with n = 6 per treatment/experiment. Asterisks indicate differences between control and stimulated cells (*P < 0.05, **P < 0.01, ***P < 0.001).