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. 2016 Nov 16;6:37095. doi: 10.1038/srep37095

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Production of total cAMP (A) and phosphorylation of AKT (B) in HUVECs and HUV-ST cells. cAMP was measured in medium collected from HUVEC and HUV-ST cells after 1 h incubation without or with 0.733, 7.33, 73.3, and 600 ng/ml of rhFSH, or 10 μM forskolin used as a positive control. Each bar represents the mean ± SEM of three independent experiments with n = 4 per treatment. Asterisks indicate differences between control and stimulated cells (***P < 0.001). Middle (B) and Lower (C) panels present the phosphorylation of AKT (pAKT) in HUVECs and HUV-ST cells. Cells starved for either 4 (B) or 8 h (C) were stimulated with 600 ng/ml of rhFSH for 15, 30 and 60 minutes. A representative picture of cropped gel has been shown here, full-length blots/gels are presented in Supplementary information file.