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. 2016 Nov 16;6:36869. doi: 10.1038/srep36869

Figure 3. PGRMC1 inhibitor AG-205 reversed the suppressive effect of P4 on primordial follicle formation in vitro.

Figure 3

For this, 16.5 dpc ovaries were isolated and were cultured with vehicle (A), 1 μM P4 alone (B), 5 μM AG-205 (PGRMC1 inhibitor) alone (C), or both AG-205 and P4 (D) for 6 days. Paraffinized sections of the ovaries were stained with anti-DDX4 (green) antibody and PI (red) for morphology analysis (A–D). Then the numbers of oocytes and follicles were counted after staining the sections with hematoxylin (E). Arrowheads indicate oocytes in cysts or naked oocytes, and arrows indicate primordial follicles; scale bar = 25 μm. Different letters denote statistical significance at P < 0.01 (ANOVA and post hoc test, n = 3–5, 3 independent replicates).