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. 2016 Nov 16;6:36983. doi: 10.1038/srep36983

Figure 5. Caspase-3 and PARP cleavage, and expression of anti-apoptotic protein Bag1 in EV71-infected RD and NSC-34 cells.

Figure 5

RD and NSC-34 cells were infected with S41, C2 and MS strains at MOI 1 and 10, respectively. At the indicated time points post-infection, Western blot analysis was performed on the cell lysates using antibodies specific to (a) full length or cleaved caspase-3 and PARP proteins or (b) anti-apoptotic protein Bag1. The band intensities were analyzed using ImageJ software, by normalizing against β-actin, and using the uninfected control (UI) as a reference (dotted line, fold change of 1). Gels images were cropped. These experiments were performed twice independently. One representative set is shown.