Table 1.

Comparison of TSSApep ELISA reactions with haemoculture and genotyping data. All samples had tested positive for T. cruzi infection with IFAT. Seven samples reacted non-specifically and are excluded (see text)

Species	Biome	Region	Haemoculture	Genotypinga	nb	TSSA peptide reaction
						II/V/VI	III	IV	V/VI	Lysatec
L. chrysomelas	Atlantic Rainforest	Una, Bahia	Positive	I	1	1	0	0	1/1 of TSSA II/V/VI	0
				II	11	8	0	0	6/8 of TSSA II/V/VI	5 (1)
				Uncharacterised	6	4	0	0	4/4 of TSSA II/V/VI	3 (1)
			Negative		8	2	0	0	1/2 of TSSA II/V/VI	5 (4)
L. chrysopygus	Atlantic Rainforest	Guapimirim, RJ	Positive	II	1	0	0	0	0	0
L. rosalia	Atlantic Rainforest	Silva Jardim, RJ	Positive	II	8 (4)	3	0	0	1/3 of TSSA II/V/VI	2
				Mixed I/II	1 (1)	1	0	0	1/1 of TSSA II/V/VI	1
				Uncharacterised	1	1	0	0	0	1
			Negative		3	3	0	0	0	3
S. libidinosus	Amazon	Estreito, Maranhão	Positive	Uncharacterised	2	0	1d	1d	0	1d
			Negative		4	0	0	0	0	0
A. bezebul	Amazon	Estreito, Maranhão	Positive	Mixed I/IV	1	0	0	0	0	0
				Uncharacterised	1	0	0	0	0	1 (1)
			Negative		5	0	0	0	0	0

^aAll samples genotyped by PCR of the mini-exon

^bParentheses indicate TcII infections confirmed by PCR:RFLP based on SNPs in the HSP60 and GPI loci and by PCR of LSU rDNA

^cParentheses indicate samples that reacted with lysate only

^dSame sample reacting with TSSApep-III and TSSApep-IV and lysate