Fig. 2.

Proportion of non-viable immature eqMoDC measured by flow cytometry. The AF488 annexin V/Dead Cell Apoptosis Kit (Invitrogen) was used for the analysis according to the protocol provided in the kit. Each symbol indicates a separate horse (n = 6), with red lines indicating the median value. A non-parametric paired sample Wilcoxon (signed rank) test was used to determine significant differences between HS and the respective FBS condition. P-values ≤ 0.05 were considered statistically significant