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. 2016 Nov 15;12:254. doi: 10.1186/s12917-016-0880-8

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Gene expression analysis of equine monocytes (Mo) and eqMoDC. Mo (dark blue), iMoDC (black, FBS batch S0113; yellow, HS) and mMoDC (light blue, FBS batch S0113; pink, HS) were generated in parallel from three horses each. Principal component analysis (PCA) was carried out on the differentially expressed genes (1331 variables) and demonstrated that while the gene expression profiles of the different MoDC stages are separate and different to Mo, the genetic profile of the different sera cannot be further separated. Three biological repeats (horses) were analyzed using Qlucore, one dataset (iMoDC, HS) was discarded due to a technical issue in the hybridization of the sub-arra