Table 2.

PCR primers used for mRNA expression analysis

Genes	Primer sequences	Acession no.	Location	Product size
IL-2	F: 5′-CTGTATTTCGGTAGCAATG-3′	NM_204153.1	87-247	161
	R: 5′-ACTCCTGGGTCTCAGTTG-3′
IL-1β	F: 5′-ACCCGCTTCATCTTCTACCG-3′	NM_204524.1	663-836	174
	R: 5′-TCAGCGCCCACTTAGCTTG-3′
IL-6	F: 5′-GGCATTCTCATTTCCTTCT-3′	NM_204628.1	853-1051	199
	R: 5′-CTGGCTGCTGGACATTTT-3′
IFN-α	F: 5′-GGACATGGCTCCCACACTAC-3′	XM_004937097.1	2896-3044	149
	R: 5′-GGCTGCTGAGGATTTTGAAGA-3′
IFN-β	F: 5′-CACCACCACCTTCTCCT-3′	NM_001024836.1	66-321	256
	R: 5′-TGTGCGGTCAATCCAGT-3′
CXCLi2	F: 5′-CATCATGAAGCATTCCATCT-3′	NM_205498.1	124-223	100
	R: 5′-CTTCCAAGGGATCTTCATTT-3′
CXCLi1	F: 5′-AACTCCGATGCCAGTG-3′	NM_205018.1	176-380	205
	R: 5′-TTGGTGTCTGCCTTGT-3′
CCR5	F: 5′-GTGGTCAACTGCAAAAAGCA-3′	NM_001045834.1	1126-1315	190
	R: 5′-GCCCGTTCAACTGTGTCG-3′
MDA-5	F: 5′-GGACGACCACGATCTCTGTGT-3′	NM_001193638.1	457-535	79
	R: 5′-CACCTGTCTGGTCTGCATGTTATC-3′
TLR3	F: 5′-GCTATTGAGCAAAGTCGAGA -3′	NM_001011691.3	2491-2693	203
	R: 5′-ACAGGGGGCACTTTACTATT-3′
TLR7	F: 5′-TCTGGACTTCTCTAACAACA-3′	NM_001011688.2	1824-2010	187
	R: 5′-AATCTCATTCTCATTCATCATCA-3′
TLR21	F: 5′-AGTTGTGTCCTGTGCTGAGAG-3′	NM_001030558.1	1035-1164	130
	R: 5′-AGCAGGTTGTGTTCCACTGTC-3′
β-actin	F: 5′- AGACATCAGGGTGTGATGGTTGGT-3′	NM_205518.1	183-300	118
	R: 5′-TGGTGACAATACCGTGTTCAATGG-3′