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. 2016 Nov 16;7:165. doi: 10.1186/s13287-016-0426-9

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — ME and DE lineage specifications. qRT-PCR was performed for the indicated genes on a time course during WJ-MSC differentiation into (a) ME, (b) DE, and (c) visceral endoderm. Differentiation was performed using the described experimental protocol described in Methods. Results were normalized to the Geo-mean of the housekeeping genes and expression relative to day 0. Data shown as mean ± SEM (n = 4). *P < 0.05