Table 2.

Steady-state kinetic parameters for the oxidation of VE dimer for wild-type and mutants

Variants	Oxidation of VE dimer
	KM (mM)	kcat (s−1)	kcat/KM (s−1 mM−1)
WT	0.13 ± 0.03	0.77 ± 0.05	5.59 ± 0.69
W251A	0.26 ± 0.01	0.06 ± 0.01	0.25 ± 0.04
W251F	0.15 ± 0.01	0.61 ± 0.08	4.10 ± 0.52
W251Y	0.16 ± 0.01	0.45 ± 0.01	2.81 ± 0.05
T208D	0.38 ± 0.01	2.44 ± 0.08	6.40 ± 0.11
A242D	0.55 ± 0.02	16.48 ± 0.20	29.96 ± 0.92
T208D/A242D	1.22 ± 0.03	16.13 ± 0.35	13.22 ± 0.31