Fig. 3.

G-CSF treatment attenuated renal infiltration of inflammatory leukocytes. a Absolute number of total leukocytes (CD45⁺) in kidney was significantly decreased in the G-CSF group (P < 0.01). b Number of infiltrating T cells (CD3⁺) including both CD4⁺ and CD8⁺ T cells was decreased in the G-CSF group (P < 0.05), whereas number of infiltrating B cells (CD19⁺) was not decreased. c Numbers of CD11b⁺ myeloid cells were lower in the G-CSF group than in the PBS group (P < 0.01). d-e However, G-CSF did not decrease renal infiltration of either CD11c⁺ dendritic cells (d) or NK cells (NK1.1⁺CD3^-, e). Flowcytometric analysis was used for quantifying renal cell types. The data are expressed as the mean ± standard error of the mean, with n = 4 − 5 mice per group. White and black bars indicate the G-CSF group and the PBS group, respectively. *P < 0.05, **P < 0.01 for comparisons between the G-CSF and the PBS groups using t-test. G-CSF: granulocyte colony-stimulating factor; PBS: phosphate-buffered saline