Fig. 5.

G-CSF treatment induced expansion of CD4⁺CD25⁺Foxp3⁺ regulatory T cells in both spleen and kidney. a Representative flow cytometric dot plots of CD25⁺Foxp3⁺ cells in kidney (CD45⁺CD4⁺ gated) and spleen (CD4⁺ gated). b-c Bar graphs showed proportions of Foxp3⁺, CD25⁺Foxp3⁺, and CD25^-Foxp3⁺ among CD4⁺ T cells or absolute numbers of CD4⁺Foxp3⁺, CD4⁺CD25⁺Foxp3⁺, and CD4⁺CD25^-Foxp3⁺ in kidneys (b) and spleen (c). The data are expressed as the mean ± standard error of the mean, with n = 4 − 5 mice per group. White and black bars indicate the G-CSF group and the PBS group, respectively. *P < 0.05, **P < 0.01 for comparisons between the G-CSF and the PBS groups using t-test. G-CSF: granulocyte colony-stimulating factor; PBS: phosphate-buffered saline; Tregs: regulatory T cells