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. Author manuscript; available in PMC: 2016 Nov 16.
Published in final edited form as: ACS Chem Neurosci. 2016 Jan 8;7(3):297–304. doi: 10.1021/acschemneuro.5b00245

Figure 3.

Figure 3

β-Arrestin2 recruitment assay using PathHunter eXpress OPRM1 CHO-K1 βarrestin2 assay kit. (A) The MOR full agonist DAMGO dose-dependently increased βarrestin2 association, whereas NAP and nalbuphine displayed no apparent agonism. (B) NAP and nalbuphine significantly antagonized DAMGO-induced βarrestin2 recruitment in a dose-dependent fashion. The IC50 (95% CI) values of NAP and nalbuphine are: 8.8 (4.0–19.3) nM and 47.2 (14.4–155.0) nM, respectively.