Fig. 3. Dynamic regulation of the ribosome recycling machinery.
A. PELO and ABCE1 are present in Meg01 cells but absent in PLPs. B. Translation factor expression in differentiating K562 cells. C. Quantification of western blots in (B), normalized to day 0. D. Schematic of recycling/rescue factor changes during K562 cell differentiation in relation to hemoglobin and 3´UTR ribosome accumulation. E. Differential expression of PELO and HBS1L isoforms in primary blood cells (PBMCs: peripheral blood mononuclear cells) with band intensities normalized to total protein. F. Quantitative mass spectrometry data from Gautier et al., 2016 showing relative protein levels during erythropoietic differentiation of primary CD34+ cells.