Table I.
Resolution and throughput of common RBC genotyping assays.
| Method | Example | Test type | Resolution | Throughput | Cases |
|---|---|---|---|---|---|
| Gel-based genotyping (PCR-RFLP, SSP-PCR, multiplex PCR) | Rh multiplex (Singleton, et al.30) | LDT | Low | Medium | 1, 2, 3, 4, 5, 6, 9 |
| BAGgene (GenTrak, Inc., Liberty, NC, USA) | RUO | Low | Medium | N/A | |
| DNA Sequencing analysis (Sanger) | Exon-specific | LDT | High | Low | 3, 6, 8 |
| Array-based genotyping | HEA BeadChip (Immucor, Norcross, GA, USA) | IVD (previously RUO) | Low | Medium (8, slides) or High (96, plates) | 1, 7, 9 |
| ID Core XT (Grifols, Los Angeles, CA, USA) | RUO | Medium | High (up to 48) | N/A | |
| RHCE and RHD BeadChips (Immucor) | RUO | Medium | Medium (8, slides) | 2, 4, 6 | |
| MALDI-TOF | HemoID (Agena, San Diego, CA, USA) | RUO | Medium | High (96 or 384) | N/A |
| cDNA analysis | RHD or RHCE cDNA analysis | LDT | High | Low | N/A |
RBC: red blood cell; PCR: polymerase chain reaction; RFLP: restriction enzyme-linked polymorphism; SSP: sequence-specific primer; LDT: laboratory-developed test; RUO: research use only; N/A: not applicable; IVD: in vitro diagnostic; MALDI-TOF: matrix-assisted laser desorption ionisation time-of-flight.