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. 2016 Feb 29;16(10):e28751. doi: 10.5812/hepatmon.28751

Figure 1. Location of Primers/Probes, and Construction of pTHC.

Figure 1.

A, Schematic representation of the HBV genome, shown in a linearized form, according to the conventional numbering of the nucleotide positions of the HBV genome. The viral rcDNA is partially double-stranded. The direct repeats, DR1 and DR2, used in the HBV replication are shown as black boxes. The location of the basal core promoter (BCP) is indicated by a black triangle. The real-time PCR primers used for the cccDNA and tDNA detection (ccc-s/ccc-as and tDNA-s/tDNA-as, respectively) are shown as small arrows. The positions of the fluorescent probes (ccc-probes and tDNA-probes) are shown as stars; B, Schematic description of the construction and structure of multistandard plasmid pTHC.