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. 2016 Nov 15;7(6):e01646-16. doi: 10.1128/mBio.01646-16

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Copyright © 2016 Danhof et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 6 — cwt1Δ and stp2Δ mutants have distinct phenotypes. Strains of the indicated genotypes (Wild-type, SC5314; stp2Δ, SVC17; cwt1Δ, SVC36; cwt1Δ stp2Δ, SVC39) were grown overnight in YPD and then diluted into YNB medium containing α-ketoglutarate (αKG) (A, B) or Casamino Acids (CAA) (C, D). (A, C) Growth was monitored by optical density. (B, D) pHs of the supernatants of the cultures whose growth is shown in panels A and C, respectively. (B) Significant differences (*, P < 0.05) were found for the cwt1Δ and cwt1Δ stp2Δ mutants relative to the WT or complemented controls at t = 6 h and t = 8 h. (C, D) The defects of the stp2Δ and cwt1Δ stp2Δ mutants in CAA medium are also significant. Error bars show standard deviations.