FIG 2 .

RPD3 and RPD31 play opposing roles in W/O switching of MTL heterozygotes. (A) Genetic deletion of RPD3 and RPD31 in MTLa/α cells showed consistent W/O switching frequencies under different culture conditions. The indicated strains were grown on YPD plates at 30°C for 2 days. Cells were plated on Lee’s glucose medium and Lee’s GlcNAc medium, respectively, and incubated at 25°C with 5% CO₂ for 5 days. (B) O/W switching of the WT (J4-2.1) and rpd3Δ/Δ and rpd31Δ/Δ mutant MTLa/α strains under different growth conditions. Pure opaque cells were plated on Lee’s glucose medium and Lee’s GlcNAc medium and cultured under normal aeration or at 25°C with 5% CO₂ for 5 days, respectively. (C and D) Comparison of W/O switching frequencies of WT, rpd3Δ/Δ, and rpd31Δ/Δ cells in MTLa/a (J130) and MTLa/α (J4-2.1) background genotypes. All strains were grown on YPD plates at 30°C for 2 days. Then cells were plated on SC-glucose medium at 25°C with 5% CO₂. After 5 days, cells from white colonies were replated on fresh SC-glucose medium at 25°C with 5% CO₂ for 5 days. *, P < 0.05, and **, P < 0.01, compared to the WT strain.