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. 2016 Oct 1;27(5):197–208. doi: 10.1089/hgtb.2016.059

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© Huan-Ting Lin et al. 2016; Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons Attribution Noncommercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.

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Figure 3. — Assay sensitivity after sample dilution with non-codon-optimized CYBB-containing gDNA. (a) To estimate the level of sensitivity of ddPCR, E91PN10 gDNA was diluted using gDNA extracted from transduced XCGD iPSCs that do not contain codon-optimized (CO) CYBB. Indicated are the concentrations of CO CYBB (dark blue) and RPP30 (green). NTC, no-template control. (b) Tabular summary of assay sensitivity. CO CYBB-non-containing gDNA was used to dilute E91PN10 gDNA samples, keeping the concentration of the reference RPP30 gene relatively constant while reducing the concentration of CO CYBB.