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. 2016 Nov 17;1(19):e91015. doi: 10.1172/jci.insight.91015

Figure 6. Role of cullin-3 in primary human aortic smooth muscle cells.

Figure 6

(A) Primary human aortic smooth muscle cells were cultured to 90% confluency. Expression of the indicated proteins in primary human aortic smooth muscle cells treated with the pan-cullin inhibitor, MLN4924 (1 μmol/l), for 24 hours. Quantification of independent samples is shown. n = 7. Error bars represent mean ± SEM. *P < 0.05, control vs. MLN4924 by Student’s t test. This is a representative of 2 independent experiments. (B) Expression of the indicated proteins in primary human smooth muscle cells after infection with adenovirus expressing GFP (AdGFP) or CUL3Δ9 (AdCUL3Δ9) or CUL3WT (AdCUL3WT) for 72 hours. Quantification of independent samples is shown. n = 7. Error bars represent mean ± SEM. *P < 0.05 by 1-way ANOVA. This is a representative of 2 independent experiments. Con, control; WT, CUL3WT; Δ9, CUL3Δ9.