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. 2016 Nov 17;1(19):e86934. doi: 10.1172/jci.insight.86934

Figure 2. ROBO2, SRGAP1, and nonmuscle myosin IIA (NMIIA) are expressed in mouse podocytes.

Figure 2

(A–C) Differentiated mouse podocytes express SRGAP1, myosin regulatory light chain (MRLC), and NMIIA heavy chain (NMHC IIA). ×400 magnification. (D) LacZ staining shows that SRGAP1 is expressed in embryonic day 16.5 mouse glomeruli (white arrow) of a Srgap1-LacZ reporter mouse kidney. ×50 magnification. (E and F) SRGAP1 protein is detected by immunofluorescence staining in mouse kidney glomeruli at postnatal day 1 (P1) and 2 weeks of age (2W). ×400 magnification. (G–I) SRGAP1 (red) is colocalized with ROBO2 (green) in the P1 mouse glomeruli as detected by coimmunolabeling. ×400 magnification. (J–L) SRGAP1 (red) is colocalized with nephrin (green) in the P1 mouse glomeruli as detected by coimmunolabeling. ×400 magnification. (M–O) SRGAP1 (red) is colocalized with podocin (green) in the P1 mouse glomeruli as detected by coimmunolabeling. ×400 magnification.