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. 2016 Nov 17;1(19):e86934. doi: 10.1172/jci.insight.86934

Figure 3. SLIT2 stimulation leads to a reduction of phosphorylated myosin regulatory light chain (p-MRLC) in differentiated podocytes.

Figure 3

Western blot assay reveals decreased p-MRLC levels in differentiated mouse podocytes after SLIT2 treatment. Cells were stimulated by media containing recombinant SLIT2 N-terminus or buffer for 10, 60, or 120 minutes before lysis. p-MRLC and total MRLC were detected by respective antibodies. Intensities of bands were measured and the ratio of p-MRLC/total MRLC was calculated. Data are presented as the mean ± SEM, n = 3. *P < 0.05 compared with its control by 2-tailed Student’s t test.